Your browser does not support JavaScript!
國立陽明大學神經科學研究所
從分子到認知
曹美玲 教授

痛覺與新藥開發研究室

曹美玲  教授(專任)
Tel:(02)2826-7153 (office),(02)2826-7000#5082 (lab)
Fax:(02)2820-0259
 
美國加州大學 舊金山分校 神經科學 博士後研究員
美國德州大學 達拉斯分校 分子及細胞生物學 博士
國立台灣大學 藥理學 碩士
國立台灣大學 藥學系 學士

 

成員

黃嘉怡 chiayea1028@gmail.com 簡肇均 am0934056578@yahoo.com.tw 

陳孟函 eric122821@gmail.com 周雅文 u9521036@gmail.com  

楊霈筠 oliviapyyang@gmail.com

 

合作研究

連正章  陽明大學神研所 教授  鄭仁坤 馬偕醫院麻醉科 主治醫師 饒瑞泓 馬偕醫院麻醉科 主治醫師

 

榮譽

指導研究生陳孟函榮獲本所碩班 2017 [105 (下)] 神經科學研究 口說競賽 第一名

指導研究生郭彥伶榮獲 2016 神研所碩班畢業論文 第一名

指導研究生郭彥伶榮獲 2016 陽明大學學術論文研討會 海報比賽佳作獎

指導研究生郭彥伶榮獲 2016 全國三分鐘生科論文口說競賽 第四名

指導研究生鄭昭甫榮獲 2015 馬偕紀念醫院跨院際聯合學術研討會 海報比賽第二名

 

研究簡介

全球有1/10人口正深受神經痛 (Neuropathic pain) 所苦。 神經痛是一群中度~重度的疼痛,一般止痛藥無效。有些嗎啡類藥物對神經痛有效,但成癮與便秘等嚴重副作用讓病人卻步。有些神經痛嗎啡亦無效,因此常令病人痛不欲生。由於神經痛的形成機制仍不清楚,故尚無有效的治療葯物。多年來我們致力於研究神經痛形成的分子機制,豐碩成果已陸續發表於國際一流期刊。同時我們也開發治療神經痛的新藥,申請專利,並與生技藥廠合作,以期造福人類。

本實驗室以鉀離子通道(potassium channels,是降低神經興奮性的關鍵元素)為研究主題。我們發現鉀離子通道大量表現於疼痛傳導的路徑上,當其表現量減少,疼痛就產生。從基因到動物行為,我們正在開發作用於鉀離子通道及其調控因子的新藥來治療神經痛。這是一個尚未被開發的新領域,值得大家一起來挖寶。

1.Development of novel drugs for neuropathic pain

2.The mechanisms of drugs for chronic pain

3.Roles of K+ channels and their modulatory proteins in nervous system

Neuropathic pain is a type of moderate to severe chronic pain resulted from nerve injury or disorder. Neuropathic pain affects 7-10% of global population, but less than half of patients report satisfactory pain relief. In addition, current available drugs have serious unwanted side effects, such sleepiness from calcium channel blockers and physical dependence from opioids. K+ channels, which act to reduce the activity of pain-sensing neurons, have been suggested to be novel drug targets for neuropathic pain. Using neuropathic pain animal models, my lab analyzes the expression and function of several K+ channels and their modulatory proteins in pain-related neurons located in the dorsal root ganglion (DRG), dorsal spinal cord and brain, from molecular/cellular to behavioral levels. Besides elucidating the mechanisms of available drugs for chronic pain, we are developing novel drugs for neuropathic pain. We are also interested in elucidating the roles of K+ channels in the developing nervous system.

Selective expression of Kv4.3 in IB4(+) nociceptors 

Kv4 channels in spinal dorsal horn neurons

 

Mirror-image pain       Intrathecal cannulation  

 

論文

Kuo YL, Cheng JK, Hou WH, Chang YC, Du PH, Jian JJ, Rau RH, Yang JH, Lien CC, Tsaur ML* (2017) K+ channel modulatory subunits KChIP and DPP participate in Kv4-mediated mechanical pain control. Journal of Neuroscience 37:4391-4404. (correspondent*)

Huang CY, Lien CC, Cheng CF, Yen TY, Chen CJ, Tsaur ML* (2017) K+ channel Kv3.4 is essential for axon growth by limiting the influx of Ca2+ into growth cones. Journal of Neuroscience 37:4433-4449.

Cheng CF, Wang WC, Huang CY, Du PH, Yang JH, Tsaur ML* (2016) Coexpression of auxiliary subunits KChIP and DPPL in potassium channel Kv4-positive nociceptors and pain-modulating spinal interneurons. Journal of Comparative Neurology 524:846-873.(correspondent*) 

Cheng CF, Cheng JK, Chen CY, Rau RH, Chang YC, Tsaur ML* (2015) Nerve growth factor-induced synapse-like structures in contralateral sensory ganglia contribute to chronic mirror-image pain. Pain 156:2295-2309. (correspondent*)

Chen YL+, Tsaur ML+, Wang SW, Wang TY, Hung YC, Lin CS, Chang YF, Wang YC, Shiue SJ, Cheng JK (2015) Chronic intrathecal infusion of mibefradil, ethosuximide and nickel attenuates nerve ligation-induced pain in rats. British Journal of Anaesthesia 115:105-111. (first authors+) 

Wang WJ, Cheng CF, Tsaur ML* (2015) Immunohistochemical localization of DPP10 in rat brain supports the existence of Kv4/KChIP/DPPL ternary complex in neurons. Journal of Comparative Neurology 523:608-628. (*:correspondent) pdf

Cheng CF, Cheng JK, Chen CY, Lien CC, Chu DC, Wang SY, Tsaur ML* (2014) Mirror-image pain is mediated by NGF produced from TNF alpha-activated satellite glia after peripheral nerve injury. Pain 155:906-920. (*:correspondent) pdf 

Huang CY,Chu D, Hwang WC, Tsaur ML* (2012) Co-expression of high-voltage-activated ion channels Kv3.4 and Cav1.2 in pioneer axons during pathfinding in developing rat forebrain. The Journal of Comparative Neurology 520:3650-3672. (*:correspondent) pdf

Chu LC +, Tsaur ML+, Lin CS, Hung YC, Wang TY, Chen CC, JK Cheng JK (2011) Chronic intrathecal infusion of gabapentin prevents nerve ligation-induced pain in rats. British Journal of Anaesthesia 106:699-705.(first authors+) pdf

Lin CF+, Tsaur ML+, Lin CS, Chen CC, Huang YJ, Cheng JK (2009) Intrathecal gabapentin does not act as an HCN channel activator in the rat formalin test. European Journal of Anaesthesiology 26:821-824. (first authors+) pdf

Chien LY, Jen-Kun Cheng JK, Chu DC , Cheng CF, Tsaur ML* (2007) Reduced expression of A-type potassium channels in primary sensory neurons induces mechanical hypersensitivity. Journal of Neuroscience 27:9855-9865. (correspondent*) pdf supplementary

Lin CS+, Tsaur ML+, Lin CF, Chen CC, Yang JR, Cheng JK (2007) Chronic intrathecal infusion of minocycline prevents the development of spinal  nerve ligation-induced pain in rats. Regional Anesthesia and Pain Medicine 32:209-216.(first authors+) pdf

Lu CL, Hsieh JC, Tsaur ML, Huang YH, Wang PS, Wu LL, Liu PY, FY Chang FY, Lee SD (2007) Estrogen rapidly modulates mustard oil–induced visceral hypersensitivity in conscious female rat: a role of CREB phosphorylation in spinal dorsal horn neurons. American Journal of Physiology-Gastrointestinal and Liver Physiology 292:G438-G446. pdf

Huang HY, Liao CW, Chen PS, Tsaur ML* (2006) Transient expression of A-type K+ channel alpha subunits Kv4.2 and Kv4.3 in rat spinal neurons during development. European Journal of Neuroscience 23:1142-1150. (correspondent*) pdf

Huang HY, Cheng JK, Shih YS, Chen PS, Wang CL, Tsaur ML* (2005) Expression of A-type K+ channel alpha subunits Kv4.2 and Kv4.3 in rat spinal lamina II excitatory interneurons and co-localization with pain-modulating molecules. European Journal of Neuroscience 22:1149-1157. (correspondent*) pdf 

Hsu YH, Huang HY, Tsaur ML* (2003) Contrasting expression of Kv4.3, an A-type K+ channel, in migrating Purkinje cells and other post-migratory cerebellar neurons. European Journal of Neuroscience 12:601-612. (correspondent*) pdf

Tsaur ML*, Wu YL, Huang FL, Shih YH (2001) Localization of an A-type K+ channel alpha subunit Kv4.2 in rat brain. Chinese Journal of Physiology 44:133-141. (correspondent*) pdf

Ju YT, Chang ACY, She BR, Tsaur ML, Hwang HM, Chao CCK, Cohen SN, Lin-Chao S (1998) Gas7: a novel gene expressed preferentially in growth-arrested fibroblasts and terminally differentiated Purkinje neurons affects neurite formation. Proceedings of the National Academy of Sciences of the United States of America 95:11423-11428. pdf

Tsaur ML*, Wan YC, Lai FP, Cheng HF (1997) Expression of B-type endothelin receptor gene during neural development. FEBS Letters 417:208-212. (correspondent*) pdf

Tsaur ML*, Chou CC, Shih YH, Wang HL (1997) Cloning, expression and CNS distribution of Kv4.3, an A-type K+ channel alpha subunit. FEBS Letters 400:215-220. (correspondent*) pdf

Stoffel M, Tokuyama Y, Trabb JB, German MS, Tsaur ML, Jan LY, Polonsky KS, Bell GI (1995) Cloning of rat KATP-2 channel and decreased expression in pancreatic islets of male Zucker diabetic fatty rats. Biochemical and Biophysical Research Communications 212:894-899. pdf

Tsaur ML, Menzel S, Lai FP, Espinosa R, Concannon P, Spielman RS, Hanis CL, Cox N.J, Beau MM, German MS, Jan LY, Bell G.I, Stoffel M (1995) Isolation of a cDNA clone encoding a KATP channel-like protein expressed in insulin-secreting cells, localization of the human gene to chromosome band 21q22.1, and linkage studies with non-insulin dependent diabetes mellitus. Diabetes 44:592- 596. pdf

Sheng M, Tsaur ML, Jan YN, Jan LY(1994) Contrasting subcellular localization of the Kv1.2 K+ channel subunit in different neurons of rat brain. Journal of Neuroscience 14:2408-2417. pdf

Sheng M, Tsaur ML, Jan YN, Jan LY (1992) Subcellular segregation of two A-type K+ channel proteins in rat central neurons. Neuron 9:271-284. PubMed

Tsaur ML, Sheng M, Lowenstein DH, Jan YN, Jan LY (1992) Differential expression of K+ channel mRNAs in the rat brain and down-regulation in the hippocampus following seizures. Neuron 8:1055-1067. PubMed

Baldwin TJ, Tsaur ML, Lopez GA , Jan YN, Jan LY (1991) Characterization of a mammalian cDNA for an inactivating voltage-sensitive K+ channel. Neuron 7:471-483. pdf

Tsaur ML, Clowes R (1989) Localization of the control region for expressionof exotoxin A in Pseudomonas aeruginosa. Journal of Bacteriology 171: 2599-2604. pdf

Lee CY, Tsai MC, Tsaur ML, Lin WW, Carlsson, FHH, Joubert FJ (1985) Pharmacological study on Angusticeps-type toxin from mamba venoms. Journal of Pharmacology and Experimental Therapeutics 233:491-498. pdf

Lee CY, Lee JL, Tsaur ML (1982) Effects of a crotamine-like toxin from Crotalus h. horridus venom on skeletal neuromuscular transmission. Journal of Formosan Medical Association 81:833-842.

 

 

 
瀏覽數  
將此文章推薦給親友
請輸入此驗證碼
Voice Play
更換驗證碼